What If My H2 Readings are Lower than Expected?

H2Blue will react with dissolved hydrogen gas and turn clear, provided that the dissolved H2 concentration is at least equal to the lower detection level of 0.1PPM.  If your device is not producing detectable dissolved H2 levels, check for the following:

1) Review tips for getting best results (see "Helpful Hints For Measuring" above)

2) Limescale (from hard water) may have accumulated on the electrodes or plumbing-  follow manufacturer's instructions for cleaning

3) Source water mineral concentrations may be too low for adequate H2 production-

 add a mineral pre-filter cartridge to boost source-water conductivity

4) The device may be malfunctioning and not producing H2 gas- contact the manufacturer    for troubleshooting procedures

5) High levels of chlorine or oxygen (such as from Brown's Gas devices) may interfere with H2Blue and give a lower-than-expected reading. Please Contact Us for more information.

6) Since H2Blue's resolution is 0.1PPM per drop, failure for even one drop to turn clear         doesn't necessarily indicate that there is no dissolved H2 in the water for a couple of             reasons:
* The water being tested could possibly have a concentration of only 0.1PPM, which would cause only one drop to remain blue. This is because we always count the last drop in the titration which causes the reagent to remain blue (in this case, the first drop would also be the last drop). But, water with no dissolved H
2 would also cause one drop to remain blue. Therefore, there is some ambiguity possible when testing levels of dissolved H2 at or near the 0.1ppm concentration. Ideally, at least one drop should clear up to verify the presence of some H2, which would in fact represent an H2 concentration of 0.2PPM.
* A concentration of less than 0.1ppm could be present, e.g. 0.05PPM, but be undetectable, since it is below the minimum detection threshold of the reagent.


Because H2Blue contains ethanol, it must be packaged and labeled according to US DOT and international IATA regulations. Because the US Postal service will not accept products containing ethanol, we must use an alternative carrier. We use Fedex Ground to ship H2Blue within the US (lower 48) and Canada. Shipping to Alaska, Hawaii and all other international addresses is via Fedex Express (air)- please contact us about orders to international locations using our Contact Page.for


H2Blue contains methylene blue, a compound used as a biological tissue dye. Therefore, H2Blue can stain any surface it touches (possibly permanently, depending on porosity). It is recommended that skin, counter-tops, floors, clothing and other surrounding surfaces be protected from inadvertent splashing during use.  The user should wear safety glasses to protect eyes from splashing. Use of a mild abrasive cleanser will usually remove stains from most surfaces. Use soap & water to remove from skin.

​​​​​Helpful Hints for Measuring 

*When measuring H2 water from water ionizers or hydrogen infusion machines, be sure to run the device for at least 20 seconds before taking the 6ml sample. This will insure that you are sampling fresh H2 water, not water which has been sitting in the machine.

*The H2Blue spout is designed to dispense a precise droplet size for testing dissolved H2 levels.  When dispensing each drop, turn the bottle completely upside down, squeeze the bottle until one drop exits the spout, then release the pressure (which creates a small vacuum). Repeat this technique for each drop. Dispensing H2Blue in this way will help to prevent excess reagent from running down the spout.

*After completing each measurement, be sure to completely rinse the beaker to remove all H2Blue residue. If H2Blue residue is permitted to accumulate on the inside of the graduated beaker, this may result in inaccurate H2 readings.

*Because dissolved hydrogen gas starts to escape immediately after the 6mL sample is taken, try to perform measurements as quickly as possible. Multiple drops of H2Blue can be added to save time, but do not add too many, which may cause you to pass the titration endpoint. Best results will be obtained when a measurement takes 1 minute or less. 

*Fill the beaker directly from the device without using any type of intermediate container such as a drinking glass, and hold the beaker at a slight angle while filling to prevent any splashing which may inadvertently force dissolved hydrogen gas out of the water.

*When doing measurements, avoid shaking the beaker vigorously, which can significantly reduce the dissolved H2. It is better to "gently swirl" or use a small straw (such as a coffee stirrer or toothpick) to slowly stir the reagent into the sample water.

*When approaching the titration endpoint, be sure to count the last drop which causes the 6mL test sample to remain pale blue.

H2Blue Safety Data Sheet Download



H2Blue is warranted to perform as described here for one year* from date of purchase. In the event that you are dissatisfied with the product, we will replace it or issue a refund at our discretion. Shipping charges are not covered by warranty and are not refundable. Please contact us using our Contact Page for more information.

*12-month Pro-Rata

 Quantity Pricing

Wholesale discount pricing in multiple tiers is available for customers wishing to purchase H2Blue in larger quantities. Please contact us using our Contact Page for more information.

H2Blue Video Tutorial

​Package Description

The H2Blue bottle contains 10 mL of reagent and will dispense approximately 400 drops (a typical test for dissolved hydrogen gas will take between 4 and 10 drops). Since bottle capacity is approximately 13 mL, it will not be completely full when received (bottles are filled to 10 mL using precision dispensing equipment). H2Blue comes in an LDPE plastic bottle with a child-resistant cap. The H2Blue bottle comes stored in a plastic graduated beaker with a screw cap. The beaker is used to measure the precise volume of hydrogen water (6 mL) required for accurate testing of dissolved Hlevels.  H2Blue ​should be stored in a cool, dry place, and should be used within 12 months of purchase.

Demonstrating the Presence of Antioxidants  

H2Blue is a reagent designed to detect the presence of dissolved hydrogen gas, the therapeutic antioxidant in ionized water (as well as in water from other devices which produce hydrogen-rich water). In the presence of dissolved hydrogen gas (H2), the molecules contained inH2Blue react with the dissolved H2 gas, and the H2Blue molecules turn "clear". When a drop ofH2Blue is added to water containing an adequate level of dissolved H2 (> 0.1ppm), the drop turns clear almost immediately.  So-called "antioxidant" bottled waters will not cause H2Blue to turn clear if they do not contain dissolved hydrogen gas. (Clear plastic bottles cannot hold hydrogen gas for longer than a few days).

​​​Measuring Dissolved Hydrogen 

H2Blue is a reagent designed to detect the presence of dissolved hydrogen gas in water by performing what chemists call a "titration". In a titration, a substance of known concentration (theH2Blue reagent) is added to a measured volume of water containing an unknown level of dissolved hydrogen gas (H2) . In the presence of dissolved hydrogen gas, the H2Blue molecules react with the dissolved H2 molecules, causing the blue molecules to turn "clear". As additional drops are added, the available dissolved hydrogen molecules are "consumed" until they are gone. This is called the "titration endpoint". When the endpoint has been reached, additional drops will no longer turn clear and the solution will remain blue. By counting the number of drops of H2Blue required to reach the titration endpoint in a 6 mL sample of water, and then multiplying the number of drops by 0.1, the level of dissolved H2 gas in parts per million (ppm) can be determined. For example, 6 drops x 0.1 = 0.6 ppm of dissolved H2​.  (see PPM Page for an explanation of parts per million)

 A Note about Methylene Blue as an Indicator

H2Blue contains a common dye, methylene blue, a compound used in biological applications. But, a solution of methylene blue by itself WILL NOT react with dissolved hydrogen. The reaction between methylene blue and H2requires the presence of a catalyst to occur. The catalyst is, by weight, the most expensive component in H2Blue.  


              Technical Information

​A portion of each sale is donated to the non-profit    Molecular Hydrogen Foundation  

which supports hydrogen education and research

 Usage Warnings

H2Blue is a laboratory-grade test reagent containing compounds not intended to be taken internally. Please download Safety Data Sheet for more information.

Insuring Peak Performance of H2 Water Devices

When hydrogen water devices are new, they may produce good levels of dissolved hydrogen gas (H2). But, as calcium contained in the source water begins to accumulate on the electrodes and hoses, the dissolved hydrogen levels can begin to drop, sometimes in as little as a few weeks. By using H2Blue to measure dissolved H2 levels, you can be sure that your device is performing at peak levels. H2Blue can also be used to measure the dissolved H2 levels in water produced by other devices including hydrogen tablets, magnesium sticks, and hydrogen-water filters.

  pH Range

TheH2Blue reagent has been tested for performance accuracy in the pH range of 4 to 9.5. Although H2Blue will still detect dissolved H2  outside of this range, results may be skewed or reaction times altered.